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carboxyfluorescein succinimidyl ester cfse  (Thermo Fisher)
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Thermo Fisher carboxyfluorescein succinimidyl ester cfse
CD16 + monocytes promoted T-cell proliferation in SLE . <t>CFSE-labeled</t> CD4 + T cells isolated from freshly collected SLE blood or blood bank collected HC blood buffy coat were cocultured with CD16 + and CD16 − monocytes for 60 h in the presence of anti-CD3 (1 μg/mL) and anti-CD 28 (1 μg/mL) antibodies and M-CSF (50 ng/mL). CFSE histograms depicted the number of events ( y -axis) and the fluorescence intensity ( x -axis), with proliferating cells displaying a progressive loss in fluorescence intensity following cell division, indicative of proliferating cells. (A) Representative histogram plots from a patient with SLE displayed the frequencies of CFSE low T cells in monocyte/T-cell cocultures and T cells cultured alone. (B) Percentages of CFSE low T cells in CD4 + T cells after coculture with each monocyte subset from five healthy donors and six patients with SLE. (C) The percentage increase in CFSE low T cells in cocultures of monocytes and T cells compared with CD4 + T cells cultured alone was compared between five healthy individuals and six patients with SLE. Data were expressed as mean ± SD and analyzed by non-parametric paired t test and Mann–Whitney U test. * P < 0.05, ** P < 0.01; NS, no significance.
Carboxyfluorescein Succinimidyl Ester Cfse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD16 + monocytes promoted T-cell proliferation in SLE . CFSE-labeled CD4 + T cells isolated from freshly collected SLE blood or blood bank collected HC blood buffy coat were cocultured with CD16 + and CD16 − monocytes for 60 h in the presence of anti-CD3 (1 μg/mL) and anti-CD 28 (1 μg/mL) antibodies and M-CSF (50 ng/mL). CFSE histograms depicted the number of events ( y -axis) and the fluorescence intensity ( x -axis), with proliferating cells displaying a progressive loss in fluorescence intensity following cell division, indicative of proliferating cells. (A) Representative histogram plots from a patient with SLE displayed the frequencies of CFSE low T cells in monocyte/T-cell cocultures and T cells cultured alone. (B) Percentages of CFSE low T cells in CD4 + T cells after coculture with each monocyte subset from five healthy donors and six patients with SLE. (C) The percentage increase in CFSE low T cells in cocultures of monocytes and T cells compared with CD4 + T cells cultured alone was compared between five healthy individuals and six patients with SLE. Data were expressed as mean ± SD and analyzed by non-parametric paired t test and Mann–Whitney U test. * P < 0.05, ** P < 0.01; NS, no significance.

Journal: Frontiers in Immunology

Article Title: CD16 + Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus

doi: 10.3389/fimmu.2016.00512

Figure Lengend Snippet: CD16 + monocytes promoted T-cell proliferation in SLE . CFSE-labeled CD4 + T cells isolated from freshly collected SLE blood or blood bank collected HC blood buffy coat were cocultured with CD16 + and CD16 − monocytes for 60 h in the presence of anti-CD3 (1 μg/mL) and anti-CD 28 (1 μg/mL) antibodies and M-CSF (50 ng/mL). CFSE histograms depicted the number of events ( y -axis) and the fluorescence intensity ( x -axis), with proliferating cells displaying a progressive loss in fluorescence intensity following cell division, indicative of proliferating cells. (A) Representative histogram plots from a patient with SLE displayed the frequencies of CFSE low T cells in monocyte/T-cell cocultures and T cells cultured alone. (B) Percentages of CFSE low T cells in CD4 + T cells after coculture with each monocyte subset from five healthy donors and six patients with SLE. (C) The percentage increase in CFSE low T cells in cocultures of monocytes and T cells compared with CD4 + T cells cultured alone was compared between five healthy individuals and six patients with SLE. Data were expressed as mean ± SD and analyzed by non-parametric paired t test and Mann–Whitney U test. * P < 0.05, ** P < 0.01; NS, no significance.

Article Snippet: For labeling isolated T cells, the cells were incubated for 10 min at 37°C in 5 mM carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen, Carlsbad, CA, USA, catalog: C34554) in phosphate-buffered saline/0.1% bovine serum albumin (BSA/0.1% PBS).

Techniques: Labeling, Isolation, Fluorescence, Cell Culture, MANN-WHITNEY